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KMID : 1161920200170020041
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Journal of Medicine and Life Science
2020 Volume.17 No. 2 p.41 ~ p.46
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Role of autophagy in metformin-induced apoptosis of H4IIE hepatocellular carcinoma cells
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Baek Keun-Ho
Park Deok-Bae
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Abstract
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Metformin, a predominantly prescribed anti-diabetic drug for decades, has gained new insights for its anti-tumor activity in a variety of cancer cells. Our previous studies also showed the obvious pro-apoptotic activity of metformin and the underlying action mechanisms in hepatocellular carcinoma cells. Together with apoptosis, autophagy is a crucial intracellular process to determine the survival or death of cells under some stressful environments. The present study aimed to determine the role of autophagy in metformin-induced death of H4IIE hepatocellular carcinoma cells. Metformin blocked the formation of autophagosomes and the expression of LC3A, generally described as biomarkers of autophagy. Inhibition of AMPK reversed the metformin-induced blockade of autophagy. Antioxidant (NAC) suppressed the metformin-induced cell death but not affected LC3A. The inhibition of protein kinase C totally restored the metformin-suppressed expression of LC3A. In summary, our present study suggests that autophagy is an anti-apoptotic player in metformin-induced apoptosis if H4IIE cells.
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KEYWORD
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Metformin, Apoptosis, Autophagy, H4IIE
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